全文获取类型
收费全文 | 3801篇 |
免费 | 301篇 |
国内免费 | 281篇 |
出版年
2024年 | 3篇 |
2023年 | 42篇 |
2022年 | 44篇 |
2021年 | 91篇 |
2020年 | 115篇 |
2019年 | 110篇 |
2018年 | 111篇 |
2017年 | 111篇 |
2016年 | 128篇 |
2015年 | 154篇 |
2014年 | 183篇 |
2013年 | 295篇 |
2012年 | 138篇 |
2011年 | 182篇 |
2010年 | 151篇 |
2009年 | 196篇 |
2008年 | 201篇 |
2007年 | 222篇 |
2006年 | 197篇 |
2005年 | 182篇 |
2004年 | 161篇 |
2003年 | 153篇 |
2002年 | 137篇 |
2001年 | 152篇 |
2000年 | 85篇 |
1999年 | 106篇 |
1998年 | 95篇 |
1997年 | 93篇 |
1996年 | 70篇 |
1995年 | 62篇 |
1994年 | 49篇 |
1993年 | 43篇 |
1992年 | 32篇 |
1991年 | 28篇 |
1990年 | 14篇 |
1989年 | 31篇 |
1988年 | 23篇 |
1987年 | 17篇 |
1986年 | 18篇 |
1985年 | 28篇 |
1984年 | 26篇 |
1983年 | 16篇 |
1982年 | 22篇 |
1981年 | 27篇 |
1980年 | 20篇 |
1979年 | 6篇 |
1978年 | 2篇 |
1977年 | 4篇 |
1976年 | 3篇 |
1973年 | 2篇 |
排序方式: 共有4383条查询结果,搜索用时 250 毫秒
991.
Hermatypic (reef building) corals live in an environment characterized by high ambient levels of photosynthetically active radiation (PAR) and ultraviolet radiation (UVR). Photoadaptive mechanisms have evolved to protect the sensitive cell structures of the host coral and their photosynthetic, endosymbiotic zooxanthellae. Environmental stressors may destabilize the coral-zooxanthellae system resulting in the expulsion of zooxanthellae and/or loss of photosynthetic pigment within zooxanthellae, causing a condition known as bleaching. It is estimated that 1% of the world’s coral population is lost yearly, partly due to bleaching. Despite intensive research efforts, a single unified mechanism cannot explain this phenomenon. Although UVA and UVB cellular damage is well documented, UVC damage is rarely reported due to its almost complete absorption in the stratosphere. A small scale coral propagation system at the University of Maine was accidentally exposed to 15.5 h of UVC radiation (253.7 nm) from a G15T8 germicidal lamp, resulting in a cumulative surface irradiance of 8.39 × 104 J m−2. An experiment was designed to monitor the progression of UVC induced damage. Branch sections from affected scleractinian corals, Acropora yongei and Acropora formosa were submitted to histopathology to provide an historical record of tissue response. The death of gastrodermal cells and necrosis resulted in the release of intracellular zooxanthellae into the gastrovascular canals. Zooxanthellae were also injured as evidenced by pale coloration, increased vacuolization and loss of membrane integrity. The recovery of damaged coral tissue likely proceeds by re-epithelialization and zooxanthellae repopulation of gastrodermal cells by adjacent healthy tissue. 相似文献
992.
993.
994.
蒽和UV—B辐射对米氏凯伦藻生长的影响 总被引:1,自引:0,他引:1
为研究多环芳烃蒽(anthracene)和UV—B辐射对米氏凯伦藻(Kreniamikimotoi Hansen)的单独效应和联合毒性效应,采用实验生态学的方法,以米氏凯伦藻为实验材料,蒽质量浓度设为0.00、6.25、11.50、20.00、35.00、62.50μg/L,UV—B辐射剂量设为0.00、0.375、1.125、2.25、3.75、6.00J/m^2。实验结果表明:对米氏凯伦藻的生长,多环芳烃蒽具有抑制效应,小剂量的UV-B具有刺激作用,随着剂量的增加表现出抑制作用,蒽与UV—B的联合则表现出更强的抑制作用,二者表现为协同作用。蒽和UV—B对米氏凯伦藻的96h—EC。分别为15.35μg/L和2.843J/m^2,而蒽在UV—B辐射条件下的96h-EC50为7.376μg/L。 相似文献
995.
Marcio R. Pie Marcel K. Tschá 《Evolution; international journal of organic evolution》2009,63(11):3023-3030
The availability of increasingly comprehensive phylogenies has provided unprecedented opportunities to assess macroevolutionary patterns, yet studies on invertebrate diversification are few. In particular, despite the ecological and evolutionary importance of ants, little is known about their tempo and mode of diversification. Recent advances in ant phylogenetics can now provide a basis for rigorous analyses of the diversification of ant lineages. The goals of the present study are threefold. First, we demonstrate that a hypothesized disproportionate increase in ant diversification during the angiosperm radiation is largely artifactual. Rather, current evidence points to a fairly constant rate of lineage growth during its history. Moreover, an analysis of diversification patterns across the ant phylogeny indicates considerable rate heterogeneity among lineages. Indeed, and contrary to the expectation if lineages had experienced a single rate of lineage increase, we found no correspondence between genus age and diversity. Finally, we demonstrate a statistically significant phylogenetic signal in ant diversification: closely related genera have diversities that are more similar to one another than one would expect by chance. This suggests that the capacity for diversification may be itself a biological trait that evolved during the radiation of the family Formicidae. 相似文献
996.
Distributional and demographic consequences of Pleistocene climate fluctuations for a marine demersal fish in the north-eastern Atlantic 总被引:1,自引:0,他引:1
Maarten H. D. Larmuseau Jeroen K. J. Van Houdt Jef Guelinckx Bart Hellemans Filip A. M. Volckaert 《Journal of Biogeography》2009,36(6):1138-1151
Aim The Pleistocene glaciations were the most significant historical event during the evolutionary life span of most extant species. However, little is known about the consequences of these climate changes for the distribution and demography of marine animals of the north‐eastern Atlantic. The present study focuses on the phylogeographic and demographic patterns of the sand goby, Pomatoschistus minutus (Teleostei: Gobiidae), a small marine demersal fish. Location North‐eastern Atlantic, Mediterranean, Irish, North and Baltic seas. Methods Analysis was carried out by sequencing the mtDNA cytochrome b gene of sand gobies from 12 localities throughout the species’ range, and using this information in combination with published data of allozyme markers and mtDNA control region sequences. Several phylogenetic methods and a network analysis were used to explore the phylogeographic pattern. The historical demography of P. minutus was studied through a mismatch analysis and a Bayesian skyline plot. Results Reciprocal monophyly was found between a Mediterranean Sea (MS) clade and an Atlantic Ocean (AO) clade, both with a Middle Pleistocene origin. The AO Clade contains two evolutionary significant units (ESUs): the Iberian Peninsula (IB) Group and the North Atlantic (NA) Group. These two groups diverged during Middle Pleistocene glacial cycles. For the NA Group there is evidence for geographic sorting of the ancestral haplotypes with recent radiations in the Baltic Sea, Irish Sea, North Sea and Bay of Biscay. The demographic histories of the Mediterranean Clade and the two Atlantic ESUs were influenced mainly by expansions dated as occurring during the Middle Pleistocene glaciations and post‐Eem, respectively. Main conclusions The pre‐LGM (Last Glacial Maximum) subdivision signals were not erased for P. minutus during the LGM. Middle Pleistocene glaciations yielded isolated and differently evolving sets of populations. In contrast to the case for most other taxa, only the northern Atlantic group contributed to the post‐glacial recolonization. The historical demography of Mediterranean sand gobies was influenced mainly by Middle Pleistocene glaciations, in contrast to that of the Atlantic populations, which was shaped by Late Pleistocene expansions. 相似文献
997.
Christopher C. Chang Oren Z. Lerman Vishal D. Thanik Carrie L. Scharf Matthew R. Greives Robert J. Schneider Sylvia C. Formenti Pierre B. Saadeh Stephen M. Warren Jamie P. Levine 《Cytokine》2009,48(3):295-302
Blood vessel growth is regulated by angiogenic and angiostatic CXC chemokines, and radiation is a vasculogenic stimulus. We investigated the effect of radiation on endothelial cell chemokine signaling, receptor expression, and migration and apoptosis. Human umbilical vein endothelial cells were exposed to a single fraction of 0, 5, or 20 Gy of ionizing radiation (IR). All vasculogenic chemokines (CXCL1–3/5–8) increased 3–13-fold after 5 or 20 Gy IR. 20 Gy induced a marked increase (1.6–4-fold) in angiostatic CXC chemokines. CXCR4 expression increased 3.5 and 7-fold at 48 h after 5 and 20 Gy, respectively. Bone marrow progenitor cell chemotaxis was augmented by conditioned media from cells treated with 5 Gy IR. Whereas 5 Gy markedly decreased intrinsic cell apoptosis (0 Gy = 16% ± 3.6 vs. 5 Gy = 4.5% ± 0.3), 20 Gy increased it (21.4% ± 1.2); a reflection of pro-survival angiogenic chemokine expression. Radiation induces a dose-dependent increase in pro-angiogenic CXC chemokines and CXCR4. In contrast, angiostatic chemokines and apoptosis were induced at higher (20 Gy) radiation doses. Cell migration improved significantly following 5 Gy, but not 20 Gy IR. Collectively, these data suggest that lower doses of IR induce an angiogenic cascade while higher doses produce an angiostatic profile. 相似文献
998.
目的研究大豆异黄酮(SI)对小鼠淋巴细胞的辐射防护作用。方法24只雄性昆明小鼠,随机分为正常对照组、辐射对照组和辐射补充0.5%SI组,喂养2周后,4.0Gy照射。照射后24 h处死小鼠,取血、胸腺和脾脏分离淋巴细胞,进行血淋巴细胞计数、观察DNA损伤情况;培养胸腺和脾脏淋巴细胞,检测3H-dT掺入量,观察淋巴细胞的增殖能力,计算淋巴细胞的增殖指数。结果辐射使小鼠胸腺和脾脏淋巴细胞数明显减少、胸腺淋巴细胞增殖能力和脾脏淋巴细胞转化指数降低、血淋巴细胞DNA损伤增加,这些变化均具有统计学意义;补充SI可降低胸腺和脾脏淋巴细胞数的减少幅度,降低胸腺淋巴细胞增殖能力和脾脏淋巴细胞转化指数下降幅度,减少辐射对血淋巴细胞DNA损伤程度,其中SI对胸腺淋巴细胞增殖能力和对血淋巴细胞DNA损伤程度的防护作用与辐射对照组相比有统计学意义。结论大豆异黄酮可对小鼠的血、胸腺和脾脏淋巴细胞有一定的辐射防护作用。 相似文献
999.
利用100 keV/μm碳离子束(初始能量为290 MeV/u)照射溶解于纯水、10 mmol/L Tris、1 mmol/L EDTA及TE 缓冲液中的pUC19质粒DNA.通过琼脂糖凝胶电泳技术分析了不同溶液中各种形态DNA分子所占份额,并计算得到不同剂量下平均每个质粒分子中单链断裂(SSB)及双链断裂(DSB)的数目.发现Tris通过抑制SSB和DSB的产生对碳重离子辐照下的质粒DNA有明显的保护作用,而EDTA能够加剧SSB的产生而抑制DSB的形成. 相似文献
1000.
小珊瑚藻对赤潮异弯藻的克生效应及其
对UV-B辐射增强的响应 总被引:1,自引:0,他引:1
通过共培养方法,以细胞密度为主要测定指标,研究了小珊瑚藻对赤潮异弯藻的克生效应及其对UV-B辐射增强的响应.结果表明:小珊瑚藻的新鲜组织和水溶性抽提液对赤潮异弯藻的生长表现出显著的抑制作用(P<0.05),说明小珊瑚藻对赤潮异弯藻具有克生效应;而小珊瑚藻干粉末和培养水过滤液对赤潮异弯藻生长则无明显影响(P>0.05).将小珊瑚藻用不同剂量的UV-B辐射预处理后,再与赤潮异弯藻共培养,除培养水过滤液外,其新鲜组织、干粉末和水溶性抽提液对赤潮异弯藻生长的作用效果均有所改变,高剂量(3.0 J·m-2)UV-B辐射使小珊瑚藻对赤潮异弯藻的抑制作用减弱(P<0.05),而低剂量(0.9 J·m-2) UV-B辐射则使抑制作用增强(P<0.05). 相似文献